How do I interpret these western blot results?

Hi! I got this western blot of the heat shock protein hsp70, and I need to interpret the result. The western is made on 2 individuals A and I and is shocked 5 minutes and 15 minutes, plus there is...

17 July 2020 167 5 View

How to use AutoDock Tools for Virtual Ligand Screening?

I downloaded the AA tranche from ZINC as a pdbqt.gz file. Following decompression of the file, can I go straight to docking using Raccoon or do I need to upload to ADT and add charges, all...

07 May 2020 3,704 1 View

Is Driscoll-Kraay standard error model approach able to handle non-stationary panel data sets?

Please, I'd like to know whether Driscoll-Kraay standard error model approach (Stata command XTSCC) able to handle non-stationary I(1) panel data sets? In other words, is this a requirement for...

17 March 2020 944 6 View

Troubleshooting beta galactose staining protocol?

Hello all! I have som trouble staining beta galactose in my MC3T3 cells. Here is my protocol: - Cells stressed with H202 treatment. - washed twice in PBS - fixated in 4% paraformaldehyde 5min...

10 July 2019 9,207 4 View

Suitable Job Stress Instruments?

Currently, I'm doing my study on the emotional intelligence and job stress among the non-academic staff. Can i know the suitable job stress instruments suitable to use?

24 April 2019 6,247 5 View

How to normalize RPKM scRNAseq data between the samples?

Do I right understand that scRNA seq data in RPKM format are normalized in the expression within one sample, but do not normalized between the samples? And, therefore, they can contain all the...

18 February 2019 8,362 2 View

Incubation Buffer for Immunoassays?

I am gonna optimise an incubation buffer for proximity extension assay (PEA), and I am wondering if I am missing anything vital in the buffer. This buffer is mainly for cell lysate detecting...

29 November 2018 684 1 View

Hi everyone, why is there a precipitate in my 0.1M CuSo4 aq solution?

I checked the pH and it is just under 4 (3.88 to be precise). It is kept in room temperature and I am wondering how can i dissolve this precipitate, which I am guessing is a Cu(OH)2. p.s. (and if...

31 October 2018 6,624 4 View

How to confirm self-firing in neurons?

Do cell-attached (CA) recordings sufficient for confirmation? I recorded in CA mode with K-gluconate intracellular solution (as after I needed to record firing patterns) without adding any...

29 April 2018 4,481 6 View

How should I prepare an RNA sample (from single cell) for further measuring of it quantaty\quality with Nanodrop?

04 September 2016 1,404 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View