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Questions related from Noemie Mermet-Joret
Hello ! I have a weak signal for Citrine attached to an AAV plasmid. I have heard that I can enhance the signal by using a GFP antibody. Any advice ?
16 April 2022 897 0 View
Good day, I prepared a K-Gluconate based internal solution which has a final osmolariry of 360 mOsm while I was expecting something around 300mOsm. It contains 170 K-gluconate, 10 HEPES,...
20 July 2020 713 0 View
Dear all, good day, I am working with a virus expressing dsRed which I injected in mouse brain, in combinaison with another one expressing EYFP. The dsRed signal is too weak to be visible under...
06 May 2020 649 2 View
Good day, We recently ran an experiment for c-fos staining within the amygdala. 90 minutes after the behavior, the animals have been transcardially perfused with PFA and the brains have been put...
09 March 2020 6,976 4 View
Good day ! I did a pilot experiment to quantify c-fos expression in amygdala after fear exposure. I had a control group with no exposure to the threat. The c-fos protocol I used was one from our...
11 October 2019 9,454 2 View
Dear all, I am interested in doing patch-clamp recording in dendrites to investigate if one segment of the dendritic tree into the amygdala receives inputs from two different regions. My question...
31 October 2017 433 2 View
Hi all, I did an immunofluorescence against c-fos in mouse's neurons using a monoclonal rabbit antibody (Cell Signaling) and a secondary Alexa 488 against rabbit (Invitrogen). I saw labeling in...
16 October 2017 9,824 9 View
Hi all of you ! Well, I am doing recordings of spontaneous EPSC for different projects, and I need to know if I can use a K-glu based internal solution (IS) to do that. I saw that this question...
08 June 2017 4,170 6 View
Hi all ! Well, I'm trying to patch cells in hippocampus organotypic slices. My pipette resistance is around 3-4 MOhm. Usually, when I patch, I have to inject a current less than -200 pA to...
25 April 2017 6,816 5 View
