12 Questions 17 Answers 0 Followers
Questions related from Noemie Mermet-Joret
Hello ! I have a weak signal for Citrine attached to an AAV plasmid. I have heard that I can enhance the signal by using a GFP antibody. Any advice ?
16 April 2022 976 0 View
Good day, I prepared a K-Gluconate based internal solution which has a final osmolariry of 360 mOsm while I was expecting something around 300mOsm. It contains 170 K-gluconate, 10 HEPES,...
20 July 2020 791 0 View
Dear all, good day, I am working with a virus expressing dsRed which I injected in mouse brain, in combinaison with another one expressing EYFP. The dsRed signal is too weak to be visible under...
06 May 2020 762 2 View
Good day, We recently ran an experiment for c-fos staining within the amygdala. 90 minutes after the behavior, the animals have been transcardially perfused with PFA and the brains have been put...
09 March 2020 7,062 4 View
Good day ! I did a pilot experiment to quantify c-fos expression in amygdala after fear exposure. I had a control group with no exposure to the threat. The c-fos protocol I used was one from our...
11 October 2019 9,539 2 View
Dear all, We recently started a couple of experiment involving red-shifted channel rhodopsin (ReaChR). First, to try our virus, we infected organotypic slices from hippocampus with ReaChR...
08 April 2018 4,338 6 View
Dear all, I am interested in doing patch-clamp recording in dendrites to investigate if one segment of the dendritic tree into the amygdala receives inputs from two different regions. My question...
31 October 2017 503 2 View
Hi all, I did an immunofluorescence against c-fos in mouse's neurons using a monoclonal rabbit antibody (Cell Signaling) and a secondary Alexa 488 against rabbit (Invitrogen). I saw labeling in...
16 October 2017 9,919 9 View
Dear colleague, I would like to stimulate my recorded cell (whole-cell, pyramidal cells in hippocampus) with a pulse train of light during 600 ms. In the middle of those pulses of light, I would...
15 June 2017 8,646 2 View
Hi all of you ! Well, I am doing recordings of spontaneous EPSC for different projects, and I need to know if I can use a K-glu based internal solution (IS) to do that. I saw that this question...
08 June 2017 4,236 6 View
Hello everyone ! I´m doing experiments in current-clamp recordings. Basically I´m trying to stimulate cells expressing an opsin sensitive to blue light to see how they respond to this stimulation...
01 June 2017 980 2 View
Hi all ! Well, I'm trying to patch cells in hippocampus organotypic slices. My pipette resistance is around 3-4 MOhm. Usually, when I patch, I have to inject a current less than -200 pA to...
25 April 2017 6,874 5 View
