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Questions related from Elina I Nagaeva
We used to use the retrobeads to mark the injection site and it did work well. But we can't use them in the current experiment, as we will perform sequencing afterwards and also cannot allow any...
02 September 2022 9,637 0 View
Do I right understand that scRNA seq data in RPKM format are normalized in the expression within one sample, but do not normalized between the samples? And, therefore, they can contain all the...
19 February 2019 8,522 2 View
Do cell-attached (CA) recordings sufficient for confirmation? I recorded in CA mode with K-gluconate intracellular solution (as after I needed to record firing patterns) without adding any...
30 April 2018 4,584 6 View
This virus is very fresh on the market and no articles have been published yet. I have never worked with hSyn promoter and wondering if it as good as CAG promoter for example? If you know any...
05 January 2018 8,095 7 View
I need to optimize my extraction procedure of RNA from single cell after patch-clamp recording. As I understood the first stage where i can measure the amount of extracted material - Nanodrop...
05 September 2016 1,524 6 View
I need to make the neuron's morphology reconstruction after patch-clamp recording. Recorded neurons are already express dTomato (red) fluorescent protein. I am going to load them during patching...
22 April 2016 5,988 12 View
Dear all! I have the problem with the displaying of the membrane voltage while putting in the current steps in current clamp mode.I have never been working with current clamp, but I suppose to see...
19 February 2016 2,662 6 View