Elina I Nagaeva

6 Questions 11 Answers 0 Followers

Questions related from Elina I Nagaeva

Which dye or marker do you use to mark the injection site when injecting with the retrograde virus?

We used to use the retrobeads to mark the injection site and it did work well. But we can't use them in the current experiment, as we will perform sequencing afterwards and also cannot allow any...

02 September 2022 9,622 0 View

How to normalize RPKM scRNAseq data between the samples?

Do I right understand that scRNA seq data in RPKM format are normalized in the expression within one sample, but do not normalized between the samples? And, therefore, they can contain all the...

19 February 2019 8,500 2 View

How to confirm self-firing in neurons?

Do cell-attached (CA) recordings sufficient for confirmation? I recorded in CA mode with K-gluconate intracellular solution (as after I needed to record firing patterns) without adding any...

30 April 2018 4,561 6 View

Did anyone tried this retro-rAAV2-hSyn-DIO-EGFP virus for retrograde Cre-dependent GFP-expression in rodents?

This virus is very fresh on the market and no articles have been published yet. I have never worked with hSyn promoter and wondering if it as good as CAG promoter for example? If you know any...

05 January 2018 8,086 7 View

How should I prepare an RNA sample (from single cell) for further measuring of it quantaty\quality with Nanodrop?

I need to optimize my extraction procedure of RNA from single cell after patch-clamp recording. As I understood the first stage where i can measure the amount of extracted material - Nanodrop...

05 September 2016 1,507 6 View

What is the best fluorescent dye for morphological reconstruction of the neuron after patch-clamp recording?

I need to make the neuron's morphology reconstruction after patch-clamp recording. Recorded neurons are already express dTomato (red) fluorescent protein. I am going to load them during patching...

22 April 2016 5,980 12 View