Hi,
I am trying to order membrane protein synthesysed gene [about 1200 bp long] to do Gibson assembly by myself because it looks like the cost for ready to express clone in using my vector is about 1000$ (Twist, Biobasic, transomic) which is a bit pricy if I need 5 clones. To order clone in their vectors (e.g. PUCvector) for subcloning also seems to be an option. I am also concerned that Twist does not verify gene fragments.
What company do you or your lab routinley use to order gene fragnment synthesys in terms of price and quality?
Thanks in advance for reply.
I synthesized 1700bp from genewiz for $460. you can choose the plasmid/vector.
Junjie Shao Hi,
How long did it take to get plasmid?
So far my best choice for bacterial gene fragments would be IDT gBlocks and BioBasic gene order if ordering 1-5 genes. My genes are about 2300 bp and the price is about 460 (CAD) each. Biobasic makes free options 20 ug of dna fragment or insert in their defolt PUC vector. Hopefully it won't take long.
if ordering 30 and more constructs I would go with codexdna 4000CAD for all tiles
Hi Elina,
I just saw your post about your gene synthesis needs. I would suggest you check out Biomatik (www.biomatik.com). We are located in Ontario, Canada, and have extensive experience in gene synthesis. We are proud of our over 98% success rate with "No Gene, No Charge" policy.
Request a quote online and we will get back to you.
Best regards,
Theresa
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