Dear all, What is the best protocol to isolate lymphocytes in skin tissue?

We want to obtain infiltrate lymphocytes in skin after Imiquimod (Aldara) treatment for FACs analysis. Some works used Liberase Blendzyme plus DNase I (Roche) and others used Dispase +...

01 February 2016 8,053 3 View

Dear all, did anyone analyzed ALCAM expression in B16 melanoma by FACs?

I'm working in the interaction ALCAM-ALCAM in metastasis models. I wondered if anyone checked the basal expression of ALCAM in B16F0 and/or B16F10. I have observed different results of ALCAM level...

01 February 2016 9,692 1 View

How long can "knockout B cell-deficient mice" survive in a conventional room?

I wonder how many time the knockout B cell-deficient mice have a normal life when change the habitat SPF to conventional room.Thank you so much for your reply!

07 August 2015 5,303 4 View

Anti-Mouse CTLA-4 to buy, where?

Does anyone know where I can purchase the antibody anti mouse-CTLA-4 to block the molecule in in vivo experiments?. I need this ab in a cheap price please. Thanks!!

09 October 2014 6,163 1 View

Does anyone know what is the cheaper kit ELISPOT of IFN-gamma (mouse) in the market?

08 September 2014 8,628 3 View

Does anybody know if in intracellular staining for study of IL-1 cytokine it is necessary to previously incubate the samples with GolgiStop?

For cytometry analysis. Thanks!

07 August 2014 6,527 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View