Have you tested the immunosuppressive function of Treg by IL-35 release with ELISA assay?
Thank you for your help!
We want to obtain infiltrate lymphocytes in skin after Imiquimod (Aldara) treatment for FACs analysis. Some works used Liberase Blendzyme plus DNase I (Roche) and others used Dispase +...
01 February 2016 8,083 3 View
I'm working in the interaction ALCAM-ALCAM in metastasis models. I wondered if anyone checked the basal expression of ALCAM in B16F0 and/or B16F10. I have observed different results of ALCAM level...
01 February 2016 9,728 1 View
I wonder how many time the knockout B cell-deficient mice have a normal life when change the habitat SPF to conventional room.Thank you so much for your reply!
07 August 2015 5,336 4 View
Does anyone know where I can purchase the antibody anti mouse-CTLA-4 to block the molecule in in vivo experiments?. I need this ab in a cheap price please. Thanks!!
09 October 2014 6,193 1 View
In my routine-work, I use BD kit ... now I would like to change for other cheaper kit. Exist others kits with the same optimal results like BD?
08 September 2014 8,668 3 View
For cytometry analysis. Thanks!
07 August 2014 6,557 5 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View
I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...
05 June 2024 8,191 0 View
I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...
03 June 2024 4,937 0 View