Dear all, What is the best protocol to isolate lymphocytes in skin tissue?

We want to obtain infiltrate lymphocytes in skin after Imiquimod (Aldara) treatment for FACs analysis. Some works used Liberase Blendzyme plus DNase I (Roche) and others used Dispase +...

01 February 2016 8,053 3 View

Dear all, did anyone analyzed ALCAM expression in B16 melanoma by FACs?

I'm working in the interaction ALCAM-ALCAM in metastasis models. I wondered if anyone checked the basal expression of ALCAM in B16F0 and/or B16F10. I have observed different results of ALCAM level...

01 February 2016 9,692 1 View

Did anyone tested the immunosuppressive function of Treg by IL-35 release?

31 December 2015 3,869 0 View

Anti-Mouse CTLA-4 to buy, where?

Does anyone know where I can purchase the antibody anti mouse-CTLA-4 to block the molecule in in vivo experiments?. I need this ab in a cheap price please. Thanks!!

09 October 2014 6,163 1 View

Does anyone know what is the cheaper kit ELISPOT of IFN-gamma (mouse) in the market?

08 September 2014 8,628 3 View

Does anybody know if in intracellular staining for study of IL-1 cytokine it is necessary to previously incubate the samples with GolgiStop?

For cytometry analysis. Thanks!

07 August 2014 6,527 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View