Does anyone know where I can purchase the antibody anti mouse-CTLA-4 to block the molecule in in vivo experiments?. I need this ab in a cheap price please.
There are a few clone available but anti-mouse CTLA-4 clone 9D9 is the one I have used for in vivo blocking (to enhance Tfh, sorry no reference its under review!). It is available from Bioxcel at fairly reasonable prices. Whether it is a blocking or depleting antibody may be a bit dependent on its microenvironment, i.e. you may find that Treg numbers are enhanced in the periphery but depleted in tumors as shown by Allison and Quezada http://jem.rupress.org/content/210/9/1695.long