Fernando Aranda

7 Questions 9 Answers 0 Followers

Questions related from Fernando Aranda

Dear all, did anyone analyzed ALCAM expression in B16 melanoma by FACs?

I'm working in the interaction ALCAM-ALCAM in metastasis models. I wondered if anyone checked the basal expression of ALCAM in B16F0 and/or B16F10. I have observed different results of ALCAM level...

02 February 2016 9,713 1 View

Dear all, What is the best protocol to isolate lymphocytes in skin tissue?

We want to obtain infiltrate lymphocytes in skin after Imiquimod (Aldara) treatment for FACs analysis. Some works used Liberase Blendzyme plus DNase I (Roche) and others used Dispase +...

02 February 2016 8,067 3 View

Did anyone tested the immunosuppressive function of Treg by IL-35 release?

Have you tested the immunosuppressive function of Treg by IL-35 release with ELISA assay? Thank you for your help!

01 January 2016 3,886 0 View

How long can "knockout B cell-deficient mice" survive in a conventional room?

I wonder how many time the knockout B cell-deficient mice have a normal life when change the habitat SPF to conventional room.Thank you so much for your reply!

08 August 2015 5,321 4 View

Anti-Mouse CTLA-4 to buy, where?

Does anyone know where I can purchase the antibody anti mouse-CTLA-4 to block the molecule in in vivo experiments?. I need this ab in a cheap price please. Thanks!!

10 October 2014 6,179 1 View

Does anyone know what is the cheaper kit ELISPOT of IFN-gamma (mouse) in the market?

In my routine-work, I use BD kit ... now I would like to change for other cheaper kit. Exist others kits with the same optimal results like BD?

09 September 2014 8,649 3 View

Does anybody know if in intracellular staining for study of IL-1 cytokine it is necessary to previously incubate the samples with GolgiStop?

For cytometry analysis. Thanks!

08 August 2014 6,541 5 View