Christoph Matti

6 Questions 7 Answers 0 Followers

Questions related from Christoph Matti

Stable cell line selection: leakiness of EF1alpha and the T2A/P2A system?

Hi all, I have a plasmid with "LTR >> EF1alphacore promoter > protein1-T2A-tagBFP-P2A-BlasticidinResistance >> LTR". I made stable murine 3T3 cells, using Blasticidin 20µg/ml,...

30 August 2023 1,022 4 View

Targeted photobleaching of Cryofixed tissue sample?

Hi all, I have tissue samples (lymph nodes) from mice, which constitutively express GFP in some cells (which I am not interested in). I usually fix and freeze the tissue in OCT. is there a way to...

23 February 2023 5,207 1 View

NP-CGG conjugation ratio?

Hi all, I am planning on NP-imunisation of mice to study B cells. However, when looking at suppliers, there are various ratio's available. Which one is recommended, or what is the rationale to...

13 January 2023 2,824 2 View

Determining Lentiviral titer without reporter cell line?

Hi all, I am transducting primary mouse splenocytes, and was wondering if there is another method to determine the quality of the (in HEK293T) produced lentivirus? As when doing a titration in HEK...

13 October 2022 3,364 1 View

Comparing different qPCR amplicons, how to account for different length of amplicons when using SyberGreen?

Dear all, I performed several qPCR, which also worked fine, however, now during the evaluation I encountered an issue. My problem is that due to the restriction when choosing primers, I ended up...

06 August 2014 3,477 2 View

For ELISA, which capture/coating AB concentration should I use?

I am developping an ELISA, which seems to work, although I am puzzled by the titration of the capture AB. The Assay: All samples were in dublicates, volume used 50ul, except 150 for blocking,...

09 April 2014 6,645 3 View