I want to isolate mesenchymal stem cell from human adipose tissue
which type of media use DMEM or DMEM-f12
and depend on what i choose my culture media for stem cell
Honestly, I asked myself the same for a long time before resolving on using the most reported medium. In brief, alpha-MEM or DMEM low glucose +10%FCS are the most reported media for MSCs expansion. If you want to stick to the standards, have a lot of patience, and have no particular requirements, use that and don't ask yourself any other question before you even start observing that the possible DMEM combinations include DMEM hi glucose, low glucose, F-12, advanced DMEM and many many others. DMEM-F12 should guarantee a faster expansion of your cells, but my suggestion would be always to test it against DMEM if you want to use it as a negative control for differentiation purposes and more quantitative analysis. If, on the other hand, are you just trying to expand your cells as fast as possible regardless on possible effects on differentiation and without knowing the medium composition, you could opt for proprietary medium such as the ones from Stem Cells Technologies or Lonza.
DMEM-F12 works quite well in our hands. It is also chipper than the specialized mediums from companies. Importantly, we use DMEM-F12 with no phenol red. Phenol red is an estrogen mimic and in cell cultures can alter cell growth and transcriptomic profile due to steroid receptor interactions.
Hello
DMEM H/L - G and DMEM/F12-based media and both used in the literature for cultures MSC . Also , the DMEM/F12 had established containing stable L-glutamine dipeptide as the optimal basal medium for propagation the MSC .
From me , was cultuerd these cells with adipogenic media containing DMEM low glucose, 10% FBS, 0.5 mM isobutyl-methylxanthine , 1 µM dexamethasone , 10 µM insulin, 200 µM indomethacin , and 1% ABAM.
Good Luck
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400011
http://www.hindawi.com/journals/sci/2013/246134/
http://www.hindawi.com/journals/bmri/2013/916136/
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