Aya Elgamal

12 Questions 3 Answers 0 Followers

Questions related from Aya Elgamal

Why does the Golgi apparatus fail to stain with H&E in a protein secreting cells?

When Golgi apparatus is stained with H&E, it appears as negative image in protein secreting cells. I want to know the reason of failure of Golgi apparatus to take H&E stain.

09 October 2022 6,473 2 View

Can anyone help me to get a protocol for cresyl violet acetate staining for cultured neural cells?

I undergo differentiation of stem cells into neural cells in 6 well plate and i want to do cresyl violet acetate staining for those cultured cells as a step to confirm differentation.

06 July 2018 9,642 7 View

Can i separate A-PRF , i-PRF and CGF with same centrifuge that separate serum and plasma or must be specific centifuge?

I read that CGF separated by medifuge while advanced PRF (A-PRF) by A-PRF centrifuge and injectable PRF (iPRF) by Duo centrifuge.But i dont have those types of centrifuge , i have that centrifuge...

18 December 2017 7,565 0 View

How can i culture mesenchymal stem cell on collagen membrane and how can confirm adherence?

i have one piece of collagen membrane (hydro-sorb F ) ( bioimplon) and i want to seed mesenchymal stem cell on it for local application in animal model , also i have granule like material and i...

18 November 2017 8,853 1 View

Can we culture stem cells without FBS?

can we culture stem cells either mesenchymal stem cell or iPSc without fetal bovine sreum during proliferetion stage not differentiation stage.

29 October 2017 7,078 8 View

What are the different methods for stem cell topical/local application in hard tissues (bone)?

I make a defect in the rat bone and i want to put stem cell in this defect as a topical application but i dont know how to implant cells without losing in blood stream ( how to inject the bone )

06 June 2017 1,370 24 View

What is the mechanism of stem cell differentiation ?

I want to Know how stem cell differentiation occur for general either in vivo or in vitro and if this mechanism differ from one cell type to another or not.

02 April 2017 4,703 3 View

What are different methods for characterization of neural cells other than pcr or immunocytochemistry ?

my research work is depend on differentiation of mesenchymal stem cells into neurons,i need specific stain for neural cells to characterize it .

03 October 2016 6,687 5 View

How to convert the fold change result into percentage in RT-PCR?

I tried to differentiate MSCs into neurons and then confirm this result by RT-PCR , the result i have is by fold change for example control sample is 1 and differentiated sample is 3 how to...

28 July 2016 3,361 1 View

What is the roles should be taken when i want to inject stem cell derived from adipose tissue in liposuction process in human clinical trial ??

i want to inject stromal cell ( stem cell ) from liposuction for human clinical trials which type of collagenase-Media-Serum- should used should i use Antibiotic or not and what type should...

23 February 2016 4,604 2 View

Depend on what i choose culture media ?

I want to isolate mesenchymal stem cell from human adipose tissue which type of media use DMEM or DMEM-f12 and depend on what i choose my culture media for stem cell

17 February 2016 1,893 4 View

Can heparin solution (ampule) replace heparin powder on cell work?

There is a paper where heparin powder is used on cells. Can I use heparin solution ampule instead? What is the difference between heparin solution and heparin powder? Usually heparin ampules...

03 December 2015 8,458 1 View