I am using silver nanoparticles, 20 wt. % (dispersion in organic solvents) (sigma, product # 719048) for an MTT assay on a HL-60 cell line and I am not getting what should be my vehicle control?
Arathi (Sesha) Paluri
Shouldn't vehicle control be organic solvent speaking in general as thats what u dispersed test item in? am not sure about purchased nanoparticles but gen if you make nanoparticles in water then that water and reactant solutions are controls (to my knowledge). Also, can you please elaborate when u said "am not getting"? Am wondering if all treated cells were dead or were the NPs seem to be non toxic?
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