The functional aspects of the massively interlinked cell rely upon efficacy of the unique NaAF controlling the Na-Pump. With such complicated function, enumeration (shown below) will be a very good way for showing multipurpose roles of the NaAF with connections to various Ca-signaling systems within a cell. However, enumeration along with a pictorial flow diagram will be the best way.
Enumeration of the distinctive roles played by the universal NaAF
1. The cytosolic NaAF (170 k Da. mass) is present in every cell of our body for the sole purpose of regulating the Na, K-ATPase (Na-pump) for homeostasis (excepting the parietal cell where an isomeric 80 k Da HAF runs both Na-pump and H-pump).
2. The NaAF starts the high-energy intermediate (E.ATP) formation along with concurrent allosteric binding of Na and K to E (E denotes the dual-topology Na, K-ATPase pump) followed by immediate hydrolysis of ATP into Pi with counter-transport of both ions to begin a new cycle.
3. The NaAF-regulated Na-pump, in turn, is handled by local Ca causing allosteric stimulation at low (< 10 µM) but abruptly (allosteric) shutting off at high (> 10 µM) Ca allowing the Na-pump to act as a provisional Ca-pump for resuming the Na-pumping.
4. Predictably, this essential NaAF self-regulates its own intracellular level through gene-expression. And, following the recent reports of auto-regulatory Ca-sensor proteins(PNAS 105, 13027–13032, 2008) in heart and brain, the NaAF may well be self-regulated with its own mRNA derived microRNAs at the translational level.
5. Being the chief operator of the Na-pump for cellular homeostasis the NaAF leads the remaining closely connected networks towards homeostasis via a cascade of Ca-signaling based holistic interactions from different parts of the cell
6. Thus, the bioenergetics demands of a cell for maintaining homeostasis following external stimuli (like hormonal signals), are promptly met by Na-sugar cotransport and Pi entry from cell exterior, for producing mitochondrial ATP to fuel the Na-pumping.
7. Hence, the Ca-signaling controlled, and NaAF-regulated allosteric Na-pump (having many tissue-specific αβ-isoforms, area-specific in brain), is a holistic function of the cell for maintaining systemic harmony via homeostasis
To complement this, a pictorial diagram of the networking processes will be ideal. Is anybody interested to join me by contributing a flow-diagram for completion of this universal theme? This will be highly exciting and helpful for all!
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