Why do they immunize mice by intraperitoneal injection?

Under what conditions will we use the method of intraperitoneal injection of vaccination in mice? In streptococcus suis research, some studies vaccinated mice by intrapetitoneal injection? why?

24 February 2018 8,500 6 View

A protocol for NKT cell depletion?

I am now planning to deplete the NKT cells in c57 mice and then injected with tumor cells. I searched online, and found some people used NK1.1, some used CD1d. I am now confusing as NK1.1 antibody...

20 December 2017 8,803 1 View

What is the maximum volume of intramuscular injection in mice?

What is the maximum volume of intramuscular injection in mice? dose translation from pig to mice?

06 September 2017 6,759 2 View

Does promoter-cre expression affect the the original gene expression?

I am confused by the Cre-flox system. For the conditional knockout (Cre-flox) system, Cre is expressed under a specific promoter. It is knocked-in using the homologue recombination, so the...

25 April 2017 9,244 3 View

What method can estimate bicycle traffic volume?

There are so many methods in estimating car traffic volume.However, methods about estimating bicycle traffic flow are very few.So I want to know some methods can estimate bicycle traffic volume.

16 March 2017 2,546 8 View

How to prepare 5ng/ml PMA from Sigma P8139?

Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...

21 February 2017 584 2 View

Which primer should I use if I want to detect the mRNA level of Akt in HCC cell lines?

Dear All, In my western blotting experiment I found a decrease level of both Akt and pAkt expression. So I want to check the mRNA level of them. I am wondering which primer should I buy for the...

16 October 2016 3,622 4 View

What is the minimum amount of starting material to use in flow-cytometry?

16 October 2016 2,440 3 View

What expression proteomics techniques to use in characterizing the protein profile of iPSC-derived leukemic cells?

06 October 2016 167 1 View

Nafion can't be used in high temperature in ORR testing, how can I solve it?

I want to coat some Pt catalyst on the glassy carbon electrode, and test it at high temperature (>140 degree). Usually, nafion is used to fix the catalyst on the GC. But as I know, nafion can't...

16 July 2016 2,190 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View