Thomas Andl

9 Questions 129 Answers 0 Followers

Questions related from Thomas Andl

What is a good way to synchronize primary human epidermal keratinocytes (NHEK)?

I was wondering whether someone has a protocol to get decent cell cycle synchronization of normal human keratinocytes in vitro. I had done a double thymidine block with HaCaT cells but I am not...

08 May 2017 7,156 4 View

Is there an in situ staining protocol for RNA content on tissue sections similar to pyronin Y?

I would like to determine whether dormant cells in situ/in vivo on tissue sections have lower RNA content. In flow cytometry pyronin Y and Hoechst or DAPI seem to be sometimes used for this...

16 August 2014 3,857 3 View

What are the absolute numbers of villi in different species?

I ran into this article claiming that the number of hematopoietic stem cells is more or less identical between rats, mice, humans and elephants DESPITE their obvious differences in size and blood...

15 August 2014 7,674 1 View

Which human tissue has the highest cancer incidence when normalized to size of the organ?

Based on the surface area of about 1.9 m2 and 3.5 million new cancers per year (Squamous cell carcinoma and basal cell carcinoma) the ectocervix has a very high cancer rate per m2 surface area:...

07 August 2014 4,281 5 View

What does Peto's paradox really mean? Are mouse tumor studies relevant?

Peto's paradox states: "There appears to be no correlation between body size, longevity and cancer across species and the absence of such a relationship is referred to as Peto’s Paradox". Example:...

06 August 2014 2,989 3 View

Where can I find skin and oral mucosa (squamous epithelia) histology pictures of long-lived mammals (whales, elephants)?

I am interested in the structure of squamous epithelia of long-lived mammals other than humans. Rodents are the main animal model for these epithelia but there are many differences between rodents...

05 August 2014 262 4 View

What is the G0 phase of the cell cycle?

Any ideas or comments on the G0 phase of the cell cycle? Are there any definitive molecular markers? I am not talking about post-mitotic, highly differentiated cells but cells that withdraw from...

13 May 2014 4,833 40 View

Has anyone heard of the "reserve cell layer" of human squamous epithelia?

I did my Ph.D. in oral tumor biology and we used the term "reserve cell layer" to describe the basal cell layer of the oral mucosa. Unfortunately, the term is rarely used nowadays and rarely finds...

13 May 2014 898 0 View

Who coined the term stem cell niche or stem cell compartment and came up with the idea that stem cells have a home?

We now use "stem cell niche" or before "stem cell compartment"all the time but this concept must have been introduced to biology by someone, somewhere, somehow.

13 May 2014 2,829 3 View