I am going to purchase the myofibroblasts of colon CCD-18 from the ATCC. I have read in the product information sheet that the cells should be defrozen inmediately upon receipt and start the culture. The recommended medium is supplied by ATCC but I have culture medium from other companies and I would like to use it. Furthermore, I worked with these cells before and I used different culture medium than recommended by ATCC.
Should I use the culture medium of ATCC to make the frozen stock?
Has somebody any experience with this? Can someone give me any advice?
Hi,
I do not have experience with these cells but if you previously used a different medium to the recommended EMEM medium and you did not observe changes in cell characteristics, then it should be OK to use the medium you want.
If you look at the ATCC technical information in the link: https://www.lgcstandards.com/WebRoot/Store/Shops/LGC/MediaGallery/catalogues/ATCC_Cell_Culture_Technical_Resource_catalogue.pdf
and go to page 8, it describes how to adapt your cells to a new medium. Good luck!
Thank you very much for your answer. I have compared the composition of my culture medium with the medium of ATCC and there is only a difference in the concentration of NaHCO3.
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