Could anybody suggest a protocol to analyse up-take of perfluorinated compounds in an intestinal cell model?

We want to investigate if perfluorinated compounds can be accumulated in our cells after exposition. Nevertheless, we have found no studies that investigate the up-take of these compounds in...

02 March 2014 670 2 View

Can anyone help with nitric oxide production in myofibroblasts of the colon?

I am analyzing the concentration of nitric oxide (NO) in culture medium after exposing myofibroblasts of colon CCD-18Co to LPS and IL-1b. As the protocol of the kit recommends to use culture...

11 December 2013 7,363 12 View

Culture of myofibroblasts of colon CCD-18.

I am going to purchase the myofibroblasts of colon CCD-18 from the ATCC. I have read in the product information sheet that the cells should be defrozen inmediately upon receipt and start the...

03 April 2013 5,023 3 View

Analysis of quecetin in tissues.

I am reading an article about the accumulation of quecetin-metabolites in the brain (Ishisaka A et al., 2011). In the analysis, the tissue is pre-treated with sulphatase and beta-glucuronidase and...

02 March 2013 8,406 0 View

Analyze miRNA expression by RT-PCR

I need to analyse the expression of miRNA and I would like to know if it is similar to the analysis of mRNA by RT-PCR. I have read in some articles that it is necessary to enrich the sample....

01 February 2013 3,024 23 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View