Juan Antonio Giménez Bastida

6 Questions 22 Answers 0 Followers

Questions related from Juan Antonio Giménez Bastida

Could anybody suggest a protocol to analyse up-take of perfluorinated compounds in an intestinal cell model?

We want to investigate if perfluorinated compounds can be accumulated in our cells after exposition. Nevertheless, we have found no studies that investigate the up-take of these compounds in...

03 March 2014 726 2 View

Can anyone help with nitric oxide production in myofibroblasts of the colon?

I am analyzing the concentration of nitric oxide (NO) in culture medium after exposing myofibroblasts of colon CCD-18Co to LPS and IL-1b. As the protocol of the kit recommends to use culture...

12 December 2013 7,423 12 View

Can someone advise on solubility of vaccenic acid in culture medium?

I am interested in treating myofibroblasts with trans-vaccenic acid and conjugated-linoleic acid. When I add vaccenic acid to the culture medium (final concentration of 50 uM) I observed black...

10 October 2013 6,634 0 View

Culture of myofibroblasts of colon CCD-18.

I am going to purchase the myofibroblasts of colon CCD-18 from the ATCC. I have read in the product information sheet that the cells should be defrozen inmediately upon receipt and start the...

04 April 2013 5,064 3 View

Analysis of quecetin in tissues.

I am reading an article about the accumulation of quecetin-metabolites in the brain (Ishisaka A et al., 2011). In the analysis, the tissue is pre-treated with sulphatase and beta-glucuronidase and...

03 March 2013 8,468 0 View

Analyze miRNA expression by RT-PCR

I need to analyse the expression of miRNA and I would like to know if it is similar to the analysis of mRNA by RT-PCR. I have read in some articles that it is necessary to enrich the sample....

02 February 2013 3,074 23 View