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Questions related from Juan Antonio Giménez Bastida
We want to investigate if perfluorinated compounds can be accumulated in our cells after exposition. Nevertheless, we have found no studies that investigate the up-take of these compounds in...
03 March 2014 728 2 View
I am analyzing the concentration of nitric oxide (NO) in culture medium after exposing myofibroblasts of colon CCD-18Co to LPS and IL-1b. As the protocol of the kit recommends to use culture...
12 December 2013 7,425 12 View
I am interested in treating myofibroblasts with trans-vaccenic acid and conjugated-linoleic acid. When I add vaccenic acid to the culture medium (final concentration of 50 uM) I observed black...
10 October 2013 6,636 0 View
I am going to purchase the myofibroblasts of colon CCD-18 from the ATCC. I have read in the product information sheet that the cells should be defrozen inmediately upon receipt and start the...
04 April 2013 5,065 3 View
I am reading an article about the accumulation of quecetin-metabolites in the brain (Ishisaka A et al., 2011). In the analysis, the tissue is pre-treated with sulphatase and beta-glucuronidase and...
03 March 2013 8,469 0 View
I need to analyse the expression of miRNA and I would like to know if it is similar to the analysis of mRNA by RT-PCR. I have read in some articles that it is necessary to enrich the sample....
02 February 2013 3,076 23 View