How is CRISPR technology a better option when compared to Restriction Enzymes , when it comes to the point of cutting a part of a DNA ? How safe and beneficial is it when it comes to the point of safety and ethical beliefs ?
Crispr and RE cannot be compared because they do entirely different things. REs cut dna at pre existing multiple recognition sites all over the genome and do nothing else . Whether you regard RE digestion as safe depends on what species of bacteria you may be. REs are designed to cut foreign dna making it more likely that the bacterium producing the RE will be free to grow well. CRISPR is targeted to an exact place on the genome and mostly anneals only once. As a technique it is neither safe nor unsafe. The result of using it may be either safe or unsafe depending on what changes are produced but it is just a method of introducing mutations of various sorts. Some of these will be good and useful and some will be dangerous and some changes will just be neutral. It is the responsibility of scientists and marketing and testing departments to sort out and reject dangerous mutants. Many aspects of life cannot easily be characterised as purely safe or unsafe and mutations frequently come into the category of sometimes safe and sometimes unsafe.
- I strongly recommend the above answer, what Mr. Paul said. Both CRISPR and restriction enzymes cut DNA at certain points. CRISPR, on the other hand, locates a specific sequence in the DNA using a guide RNA. The guide RNA is usually longer than the restriction enzyme motifs, implying that they will bind to more distinct sequences compared to restriction enzymes. However, you know some common features can be observed in both techniques; Part of CRISPR/Cas9 and restriction enzymes are endonucleases, Both CRISPR and restriction enzymes are sequence-specific. The main advantage of using CRISPR is its precise sequence targeting compared to restriction enzymes. Restriction enzyme is just an axe cutting DNA into pieces where the target sequence found but CRISPR is a laser scalpel which targets more unique location.... However, science is curious may have some side effects on it as Mr. Paul said. Manipulation in a right manner is your duty.
They are too Different. RE just cut sticky or blunt end for ligation of another sequence inside. Crispr has a wide range of application not only to cut and knock out the genes but also it used as genome editing tool and can enhance or inhibit gene expression ( catalytically dead cas9). Furtherly used in tracking the spatial arrangement of sequences. In conclusion, crispr is very flexible technology and we're modified for different purposes.
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