Could you tell me which kit is good for DNA extraction from Ganoderma lucidum mycelium?

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Can increasing the culture medium for streptomyces from 200 mL to 400 mL in 1000 mL flask affect the metabolic profile especially the main peaks ?

based on hands-on experience, could you answer my question. I have made small trials for applying some factor to induce the production of some enzymes that biotransform a substrate of interest...

22 February 2024 8,512 2 View

Why sequencing failed several times by using degenerative primers although the gel electropheresis shows bright and thick bands?

I sent a gene i recovered from gel after I amplified the gene from the plasmid extracted from metagenomic library by degenerative primers to a sequencing company but the sequencing failed several...

29 June 2023 7,596 4 View

Which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

23 February 2019 7,882 3 View

Are basidiospores of Ganoderma lucidum can be grown asexually into a mycelial stage on PDB or not because i need to use it as a homogenous inoculum ?

in fact , it is proplematic to homogenize mycelia of Ganoderma lucidum perfectly and I need a homogenous inoculum in an experiment with dry weight as a result. and so i need to have a homogenous...

11 February 2019 5,783 0 View

Kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculation of CYM in stress experiemnt for 9 minutes and unxpectedly ?

kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculationon CYM liquid media in stress experiment. and I wanted to make sure that my inoculum is homogenous so i used a...

06 January 2019 8,755 0 View

How to obtain a concentration of 0.021g CH4 per kg H2O given that it was prepared by a flow of 160 ml/min methane gas from methane cylinder in d H2O?

how to obtain a concentration of 0.021 g CH4 per kg H2O given that it was prepared by a flow of 160 ml/min methane gas from methane cylinder into d H2O in an experiment on plants. but, i am...

27 December 2018 3,894 0 View

If i applied heat stress on a fungus with differnt time 30, 60 , 90, 120 minutes. and the inhibition of mycelial growth incresed with time increase ?

and i am going to apply a substance to alleviate this stress. So, which treatment time of stress should i treat with alleviating substance. i have read that 50% inhibition dose are used in...

11 December 2018 4,817 0 View

IS centrifugation for fungal mycelia is an appropraite method for homogenization or there is a better method given we dont have a blender in the lab?

IS centrifugation for fungal mycelia is an appropraite method for homogenization or there is a better method given we dont have a blender in the lab

24 November 2018 2,615 0 View

How to get mycelium of Trichoderma in culture media?

Currently working on trichoderma spp., but getting only spores in media. How to grow and observe trichoderma mycelium in PDA or other culture media. Kindly help.

14 December 2023 8,283 2 View

Why does the culture of Ganoderma on PDA preserved in refrigerator not growing?

We grew a wild variety of Ganoderma by tissue culture on PDA. The pure culture of the isolate was preserved in a 4C refrigerator. Surprisingly, after two weeks of preservation when we tried to...

12 November 2023 2,506 1 View

What phenotypic characteristics distinguish Streptomyces coelicolor and Streptomyces lividans from each other?

Aerial mycelium color on ISP media? Utilization of carbon source?

07 November 2023 7,363 2 View

Assessment of variables that influence Ganoderma Basal Stem Rot disease outbreak in oil palm area using Spatial Modelling?

Can anyone provide more details on the type of epidemiological, meteorological and geological data that have influence the basal stem rot disease, the variables included, and the specific analysis...

07 November 2023 9,008 0 View

Why must fungal mycelium be isolated on agar before DNA extraction for PCR?

I will be collecting Armillaria spp. field samples for identification to species level using PCR. These field samples will be rhizomorphs, mycelium lifted from infected trees and in some cases...

15 October 2023 8,242 4 View

How to calculate surface mycelium density in mushroom cultivation ?

How to read or measure the surface mycelium density of the mushroom.

30 August 2023 5,384 0 View

How should I prepare a liquid culture of filamentous fungi for an inhibition assay?

Our lab develops antifungal drugs. I'm developing an assay for detecting the effect of antifungal compounds on the growth of Colletotrichum dematium. My plan is to grow liquid cultures of C....

27 June 2023 604 6 View

I would like to know if there is a specific liquid culture medium to grow Trichoderma harzianum in a research laboratory. Can I use liquid PDA?

I will need to retrieve the mycelium from the fungus to perform an RNA extraction

11 June 2023 2,248 9 View

What is the most appropriate statistical test for measuring the interaction of factors?

Hello, I'm a master student working with fungi. And one of my studies includes the evaluation of the mycelium growth efficiency and biomass production of mushroom strains on different culture...

07 May 2023 1,304 14 View

How to determine the K and a value of a polymer solvent sytem?

We are trying to determine the molecular weight of a polysaccharide extracted from Ganoderma sp. by using the Mark-Houwink equation. However, I cannot seem to find the K and a values needed to use...

04 April 2023 7,136 1 View

Rodrigo Cardoso de Oliveira

I`ve been using Prepman (Promega) and also DNAeasy kit for fungal DNA extractions with good purity and efficient amplification.

Feodora Grace Japanis

I've been using CTAB method for fungal DNA extraction. I usually have purity ranging 1.8 to 2.0 and concentration up to 1000ng/uL. It all boils down on how careful you are during the phenol:chloroform:isoamyl alcohol step.