Could you tell me which kit is good for DNA extraction from Ganoderma lucidum mycelium?

Easy and effective method to visualize fungal mycelium inside leaf tissue?

Is there any easy and efficient method to visualize the fungal mycelium inside infected leaf tissue using light microscoopy? Please share protocols which can be performed using limited resources...

03 January 2021 4,217 2 View

How can identify a fungal isolate with out RNA or ITS Seq?

Deaer Researchers, I have isolated 20 to 30 fungal isolates from fruit and now have pure colonies . so i want to identify them on morphologically which fungi are those, some isolates...

11 December 2019 2,657 5 View

Long-term storage of Alternaria spp?

I'm currently isolating a number of Alternaria solani and A. alternata strains from leaf samples and am considering my options for long-term storage of both mycelium and spores. I've read...

29 October 2019 5,982 3 View

What is a suitable way to extract phytophthora mycelium from liquid media?

The mycelium nearly completly covers the whole 'pea-media'. The strain has been growing at 28oC, not shaking.

28 October 2019 6,549 4 View

What is a suitable way to extract phytophthora mycelium from liquid media?

The mycelium nearly completly covers the whole 'pea-media'. The strain has been growing at 28oC, not shaking.

09 October 2019 3,098 5 View

I am facing problem during breaking cell wall of fungi (wood decay fungus) through sonication. Is there any other best method to break the cell wall?

I also grinded mycelium in liquid nitrogen through motar and pistal. Explain Please.

05 June 2019 7,485 4 View

What is the species of fungi?

What is the species of fungi? Growing on Sabouraud. Color of mycelium is brown.

04 May 2019 6,152 1 View

How I can make a clean separation of fresh mycelium and culture medium on Petri dishes, to get fresh mycelium weight without medium influence?

I have some Pleurotus eryngii on Petri dishes with alternative solid culture medium and need to get fresh mycelium weight without the medium weight influence, which are the best and efficient...

09 April 2019 345 6 View

Which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

01 February 2019 1,780 4 View

How I can get spore from Isaria Javanica?

I am growing this specific strain on SDAY @ 25-27C, dark condition as suggested from most papers and websites found. However, first it was producing very good amount of spores but now this...

31 December 2018 8,437 0 View

Rodrigo Cardoso de Oliveira

I`ve been using Prepman (Promega) and also DNAeasy kit for fungal DNA extractions with good purity and efficient amplification.

Feodora Grace Japanis

I've been using CTAB method for fungal DNA extraction. I usually have purity ranging 1.8 to 2.0 and concentration up to 1000ng/uL. It all boils down on how careful you are during the phenol:chloroform:isoamyl alcohol step.