Dyaaaldin Abdalmegeed

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Questions related from Dyaaaldin Abdalmegeed

Can increasing the culture medium for streptomyces from 200 mL to 400 mL in 1000 mL flask affect the metabolic profile especially the main peaks ?

based on hands-on experience, could you answer my question. I have made small trials for applying some factor to induce the production of some enzymes that biotransform a substrate of interest...

23 February 2024 8,498 2 View

Why sequencing failed several times by using degenerative primers although the gel electropheresis shows bright and thick bands?

I sent a gene i recovered from gel after I amplified the gene from the plasmid extracted from metagenomic library by degenerative primers to a sequencing company but the sequencing failed several...

30 June 2023 7,580 4 View

Which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

24 February 2019 7,867 3 View

Are basidiospores of Ganoderma lucidum can be grown asexually into a mycelial stage on PDB or not because i need to use it as a homogenous inoculum ?

in fact , it is proplematic to homogenize mycelia of Ganoderma lucidum perfectly and I need a homogenous inoculum in an experiment with dry weight as a result. and so i need to have a homogenous...

12 February 2019 5,773 0 View

Kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculation of CYM in stress experiemnt for 9 minutes and unxpectedly ?

kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculationon CYM liquid media in stress experiment. and I wanted to make sure that my inoculum is homogenous so i used a...

07 January 2019 8,739 0 View

How to obtain a concentration of 0.021g CH4 per kg H2O given that it was prepared by a flow of 160 ml/min methane gas from methane cylinder in d H2O?

how to obtain a concentration of 0.021 g CH4 per kg H2O given that it was prepared by a flow of 160 ml/min methane gas from methane cylinder into d H2O in an experiment on plants. but, i am...

28 December 2018 3,879 0 View

If i applied heat stress on a fungus with differnt time 30, 60 , 90, 120 minutes. and the inhibition of mycelial growth incresed with time increase ?

and i am going to apply a substance to alleviate this stress. So, which treatment time of stress should i treat with alleviating substance. i have read that 50% inhibition dose are used in...

12 December 2018 4,803 0 View

IS centrifugation for fungal mycelia is an appropraite method for homogenization or there is a better method given we dont have a blender in the lab?

IS centrifugation for fungal mycelia is an appropraite method for homogenization or there is a better method given we dont have a blender in the lab

25 November 2018 2,600 0 View

Could you tell me which kit is good for DNA extraction from Ganoderma lucidum mycelium?

it's my first time to extract DNA from ganoderma lucidum mycelium and I have read that CTAB method doesn't give pure DNA and there is A kit called DNAeasy kit that is good.

14 November 2018 2,916 2 View