Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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23 February 2021 4,014 3 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the minimum centrifugation RPM and minimum time for TiO2 nanoparticle synthesis from plant extract?

In my laboratory, I have an analog centrifuge (does not show real RPM value). Its' brochure indicates the range is 300-3000 RPM. The regulator is marked from 1 to 10. Is there any way to determine...

01 February 2021 2,061 4 View

Does anyone have experience with NCI H716 cell line?

Hello! I am working with NCI H716 cells and I am struggling a bit trying to "wash" the cells since they are producing significant debris. I have centrifugated and resuspended them in their media...

28 January 2021 6,421 1 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

Can we re-freezing of brain tissue homogenates?

Hello! We use homogenates of mouse brains for our aims (determination of cytokines by the ELISA method). After removing the brain, we freeze the tissue in liquid nitrogen, then store it for a...

19 January 2021 5,203 1 View

Density homogenization in Multiscale modelling?

Hello, For now, I am following the integrated technique to derive the mass from the smaller scale (microstructure). Is there any better approach to get the mass of a given macro-structure from...

16 January 2021 4,792 1 View