25 November 2018 0 3K Report

IS centrifugation for fungal mycelia is an appropraite method for homogenization or there is a better method given we dont have a blender in the lab

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Can increasing the culture medium for streptomyces from 200 mL to 400 mL in 1000 mL flask affect the metabolic profile especially the main peaks ?

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Why sequencing failed several times by using degenerative primers although the gel electropheresis shows bright and thick bands?

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Which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?

23 February 2019 7,882 3 View

Are basidiospores of Ganoderma lucidum can be grown asexually into a mycelial stage on PDB or not because i need to use it as a homogenous inoculum ?

in fact , it is proplematic to homogenize mycelia of Ganoderma lucidum perfectly and I need a homogenous inoculum in an experiment with dry weight as a result. and so i need to have a homogenous...

11 February 2019 5,783 0 View

Kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculation of CYM in stress experiemnt for 9 minutes and unxpectedly ?

kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculationon CYM liquid media in stress experiment. and I wanted to make sure that my inoculum is homogenous so i used a...

06 January 2019 8,755 0 View

How to obtain a concentration of 0.021g CH4 per kg H2O given that it was prepared by a flow of 160 ml/min methane gas from methane cylinder in d H2O?

how to obtain a concentration of 0.021 g CH4 per kg H2O given that it was prepared by a flow of 160 ml/min methane gas from methane cylinder into d H2O in an experiment on plants. but, i am...

27 December 2018 3,894 0 View

If i applied heat stress on a fungus with differnt time 30, 60 , 90, 120 minutes. and the inhibition of mycelial growth incresed with time increase ?

and i am going to apply a substance to alleviate this stress. So, which treatment time of stress should i treat with alleviating substance. i have read that 50% inhibition dose are used in...

11 December 2018 4,817 0 View

Could you tell me which kit is good for DNA extraction from Ganoderma lucidum mycelium?

it's my first time to extract DNA from ganoderma lucidum mycelium and I have read that CTAB method doesn't give pure DNA and there is A kit called DNAeasy kit that is good.

13 November 2018 2,931 2 View

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