IS centrifugation for fungal mycelia is an appropraite method for homogenization or there is a better method given we dont have a blender in the lab
based on hands-on experience, could you answer my question. I have made small trials for applying some factor to induce the production of some enzymes that biotransform a substrate of interest...
22 February 2024 8,512 2 View
I sent a gene i recovered from gel after I amplified the gene from the plasmid extracted from metagenomic library by degenerative primers to a sequencing company but the sequencing failed several...
29 June 2023 7,596 4 View
kindly please, which is better, making serial dilution for Ganoderma lucidum fungus in its PDB medium or in saline . and if saline is better , WHICH type of saline ?
23 February 2019 7,882 3 View
in fact , it is proplematic to homogenize mycelia of Ganoderma lucidum perfectly and I need a homogenous inoculum in an experiment with dry weight as a result. and so i need to have a homogenous...
11 February 2019 5,783 0 View
kindly. i tried to homogenize the liquid seed of ganoderma lucidum for inoculationon CYM liquid media in stress experiment. and I wanted to make sure that my inoculum is homogenous so i used a...
06 January 2019 8,755 0 View
how to obtain a concentration of 0.021 g CH4 per kg H2O given that it was prepared by a flow of 160 ml/min methane gas from methane cylinder into d H2O in an experiment on plants. but, i am...
27 December 2018 3,894 0 View
and i am going to apply a substance to alleviate this stress. So, which treatment time of stress should i treat with alleviating substance. i have read that 50% inhibition dose are used in...
11 December 2018 4,817 0 View
it's my first time to extract DNA from ganoderma lucidum mycelium and I have read that CTAB method doesn't give pure DNA and there is A kit called DNAeasy kit that is good.
13 November 2018 2,931 2 View
- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...
03 August 2024 2,366 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
isolation of microplastic from sludge sample using centrifugation ..
23 July 2024 6,418 0 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
01 July 2024 7,007 1 View
For Polybutylene Terephthalate (PBT) it is known that if you cool it down at fast rates to have an additional melting Peak in DSC-Scans. Androsch et al. (2015) conclude that this is due to...
30 June 2024 2,891 1 View
Hi... I seem to be in a very big trouble. My His columns look cracked after my last purification. In another column, the materials seem to be pushed. How is it possible and is there anything I...
24 June 2024 7,071 2 View
We are trying to use flow cytometry to detect cell viability / apoptosis to determine IC50 values after exposing adherent cells to drugs. We are concerned that during the washing / processing...
23 June 2024 4,279 0 View