Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to efficiently precipitate platinum(IV) prodrugs from DMSO?

I am working with platinum(IV) prodrugs. Most of the synthetic procedures states that after most of DMSO is removed the residue should be treated with methanol and ether, giving the desired...

12 July 2020 1,577 3 View

What scale would you recommend to measure patient satisfaction with using innovative service?

I'm looking for a validated measurement tool, which could be used to assess clients' satisfaction with using an innovative service. Targeted user group - patients with mental health disorders.

04 June 2019 3,442 4 View

What kinds of lateral grain distributions exist?

We're now preparing an article about MRI contrasting by magnetitenanoparticles and I'm puzzled, what kind of grain statistic can be usedto describe non-uniformity of lateral particle distribution....

10 September 2016 2,081 1 View

Where can I get this work: Dirichlet P.G.L. Abh. der Koniglich. Preus. Acad. der Wiss. , 1850?

14 April 2016 8,026 3 View

Can I seed H295R cells one cell in one well in a 96-well plate?

I am afraid that 1 cell alone would survive very difficult. Any recommendations?Should I use a medium rich in growth factors? To use a mix of old and new mediums? eg 50% of old medium and 50% of...

25 March 2015 792 3 View

What is the nonlinear refractive index of polycarbonate?

When calculating the critical power of self-focusing is requaired nonlinear refractive index, but my search didn't lead to success. Is there any information about it value?

03 December 2014 7,946 6 View

Does anyone know how to reliably differentiate between adrenal adenoma from metastatic RCC in adrenal gland?

IHC, EM or simple histology - everything is OK, but I need 100% of assurance.

11 October 2014 4,637 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View