Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to efficiently precipitate platinum(IV) prodrugs from DMSO?

I am working with platinum(IV) prodrugs. Most of the synthetic procedures states that after most of DMSO is removed the residue should be treated with methanol and ether, giving the desired...

12 July 2020 1,577 3 View

What scale would you recommend to measure patient satisfaction with using innovative service?

I'm looking for a validated measurement tool, which could be used to assess clients' satisfaction with using an innovative service. Targeted user group - patients with mental health disorders.

04 June 2019 3,442 4 View

What kinds of lateral grain distributions exist?

We're now preparing an article about MRI contrasting by magnetitenanoparticles and I'm puzzled, what kind of grain statistic can be usedto describe non-uniformity of lateral particle distribution....

10 September 2016 2,081 1 View

Where can I get this work: Dirichlet P.G.L. Abh. der Koniglich. Preus. Acad. der Wiss. , 1850?

14 April 2016 8,026 3 View

Can I seed H295R cells one cell in one well in a 96-well plate?

I am afraid that 1 cell alone would survive very difficult. Any recommendations?Should I use a medium rich in growth factors? To use a mix of old and new mediums? eg 50% of old medium and 50% of...

25 March 2015 792 3 View

What is the nonlinear refractive index of polycarbonate?

When calculating the critical power of self-focusing is requaired nonlinear refractive index, but my search didn't lead to success. Is there any information about it value?

03 December 2014 7,946 6 View

Does anyone know how to reliably differentiate between adrenal adenoma from metastatic RCC in adrenal gland?

IHC, EM or simple histology - everything is OK, but I need 100% of assurance.

11 October 2014 4,637 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View