Is it possible to test siRNA transfection by PCR?

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Robert Adolf Brinzer

You would need to use QPCR. Yes you do need to check how much knockdown you are getting with your siRNA.

Raoudha Missoum

it's possible to do an RTqPCR in order to see the KO of your RNA, using the adequate controle

Daniil Plevako

Raoudha Missoum Thank you for your answer! Do you mean i have to use a scramble siRNA as a "-" controle?

I haven't used a scramble siRNA but i used 2 housekeeping genes at the same time (GAPDH & ActinB)