Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to efficiently precipitate platinum(IV) prodrugs from DMSO?

I am working with platinum(IV) prodrugs. Most of the synthetic procedures states that after most of DMSO is removed the residue should be treated with methanol and ether, giving the desired...

12 July 2020 1,577 3 View

What scale would you recommend to measure patient satisfaction with using innovative service?

I'm looking for a validated measurement tool, which could be used to assess clients' satisfaction with using an innovative service. Targeted user group - patients with mental health disorders.

04 June 2019 3,442 4 View

What kinds of lateral grain distributions exist?

We're now preparing an article about MRI contrasting by magnetitenanoparticles and I'm puzzled, what kind of grain statistic can be usedto describe non-uniformity of lateral particle distribution....

10 September 2016 2,081 1 View

Where can I get this work: Dirichlet P.G.L. Abh. der Koniglich. Preus. Acad. der Wiss. , 1850?

14 April 2016 8,026 3 View

Can I seed H295R cells one cell in one well in a 96-well plate?

I am afraid that 1 cell alone would survive very difficult. Any recommendations?Should I use a medium rich in growth factors? To use a mix of old and new mediums? eg 50% of old medium and 50% of...

25 March 2015 792 3 View

What is the nonlinear refractive index of polycarbonate?

When calculating the critical power of self-focusing is requaired nonlinear refractive index, but my search didn't lead to success. Is there any information about it value?

03 December 2014 7,946 6 View

Does anyone know how to reliably differentiate between adrenal adenoma from metastatic RCC in adrenal gland?

IHC, EM or simple histology - everything is OK, but I need 100% of assurance.

11 October 2014 4,637 3 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View