Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to efficiently precipitate platinum(IV) prodrugs from DMSO?

I am working with platinum(IV) prodrugs. Most of the synthetic procedures states that after most of DMSO is removed the residue should be treated with methanol and ether, giving the desired...

12 July 2020 1,577 3 View

What scale would you recommend to measure patient satisfaction with using innovative service?

I'm looking for a validated measurement tool, which could be used to assess clients' satisfaction with using an innovative service. Targeted user group - patients with mental health disorders.

04 June 2019 3,442 4 View

Is there a way to determine stability constant of an element - sorbent system (Resin) trough distribution coefficients or a gamma spectroscopy?

Hello, for already determined distribution coefficients in different systems aqueous solution/resin, via gamma spectroscopy, Is there a way to determine a stability constant in order to...

27 February 2018 1,414 3 View

What kinds of lateral grain distributions exist?

We're now preparing an article about MRI contrasting by magnetitenanoparticles and I'm puzzled, what kind of grain statistic can be usedto describe non-uniformity of lateral particle distribution....

10 September 2016 2,081 1 View

Where can I get this work: Dirichlet P.G.L. Abh. der Koniglich. Preus. Acad. der Wiss. , 1850?

14 April 2016 8,026 3 View

Can I seed H295R cells one cell in one well in a 96-well plate?

I am afraid that 1 cell alone would survive very difficult. Any recommendations?Should I use a medium rich in growth factors? To use a mix of old and new mediums? eg 50% of old medium and 50% of...

25 March 2015 792 3 View

What is the nonlinear refractive index of polycarbonate?

When calculating the critical power of self-focusing is requaired nonlinear refractive index, but my search didn't lead to success. Is there any information about it value?

03 December 2014 7,946 6 View

Does anyone know how to reliably differentiate between adrenal adenoma from metastatic RCC in adrenal gland?

IHC, EM or simple histology - everything is OK, but I need 100% of assurance.

11 October 2014 4,637 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

How to increase DPI in my image?

I'm submitting manuscript to a journal that requires 1200 dpi in my images. I have changed the dpi from 96 but when I upload the image with 1200 dpi, the image looks super blurry when I review my...

16 February 2021 2,803 3 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Gain and offset values in Sentinel-2 L1C?

Hello everyone. I have been trying to apply radiometric calibration on Sentinel-2 L1C images (bands 2,3,4 and 8), but every time after layerstacking I come across this error: "Calibration...

28 January 2021 7,160 1 View

How can you confirm new liquid FTIR cell fits calibration?

We have an FTIR instrument which was calibrated in transmission/absorption mode using a set of cells for different ranges of D2O concentration. One of the cells at high range broke and the new one...

16 January 2021 4,791 2 View

Standard addition calibration without blank matrix available?

I have a problem of analytical chemistry. We are working on a spectroscopy technique (FTIR) for the quantification of a mineral phase in complex matrices. We intended to use chemometric techniques...

12 January 2021 7,739 1 View

Could anyone suggest a thermal insulation spray/coating/paint which can resist more 350 degree celsius ?

Required a spray or paint which can resist the heat flow between two steel block in physical contact. Working temperature is 250 degree celsius.

12 January 2021 2,206 6 View

How does the dilution of matrix-match calibrators impact the ionization in LC-MS/MS?

Greetings. I would like to get opinions from the experts for the improvement and optimization of multiple drug confirmation by LC-MS/MS. 1. One of our method calibration curves follows the...

10 January 2021 9,559 2 View