Hi Everyone,

So I am trying to to do a Western blot from mouse spleen lysates of four different genotypes. With actin I get plenty of background whereas GAPDH works better. The problem is that in the two control genotypes I get a good amount of GAPDH but in the two experimetnal genotypes the GAPDH is greatly reduced. I am not sure whether this was an overestimation of the protein quantity or it is a genotype thing (I do have increased apoptosis). I run an SDS-PAGE and stained it by coomasie and to my understanding I load equal amount of protein. Can you suggest another housekeeping gene to look up. See the attached file for details pelase