I have encountered a potential problem with filter sterilization of supernatants from Enterococcus strains, which according to PCR data, are hosts of several multiple bacteriocin genes, but show very little activity against Listera. Does anyone have practical experience with absorption of bacteriocins, and especially enterocins, on filter materials like cellulose acetate, PVDF, PES etc?
Dear Panagiotis Chanos,
The type of filter is not effect on bacteriocin activity. I was use the cellulose acetate filter for pediocin purification.
I suggest you to use whey as media culture for bacteriocin production.
Many the reasons for low inhibition activity of bacteriocins.
In our case, we use a hydrophilic 0,2 µm membrane filter (Surfactant-free cellulose acetate). Try to purify your Enterocin by ammonium sulfate precipitation CFS.
the following article may be helpful:
Article Characterization of semipurified enterocins produced by Ente...
All the best,
I have encountered the same situation after pH dependant method but guess the ph which is 7 is not suitable for bacteriocin activity. purification always affect the activity but not filtration. to release the bacteriocin from cells, you have to follow the ph acidity degree responsible for adsorption and release the bacteriocin from cells. or simply use am. sulphate
Thank you all for your answers. I am aware about the pH dependent absorption of bacteriocin to producer cells according to the articel from Yang (2000). However I have been researching a little more and I see that Enterocins A and B are actually more active at pH 6.5 rather than at lower pH. Still no idea though how they behave against filtering material as well as plasticware and glassware.
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