Panagiotis Fotakis

3 Questions 3 Answers 0 Followers

Questions related from Panagiotis Fotakis

Hi all. I would like to load macrophages with cholesterol using POPC/cholesterol liposomes. Any advice on the ratio and preparation method? Thanks

I have used DMPC cholesterol so far but I would preper POPC/cholesterol

09 July 2017 2,219 0 View

Safe concenctration of Guinidine hydrochloride in cell cultures?

Hello, I need to use guinidine hydrochloride to clear any aggregates of apoA-I in my solution and then treat cells with it. What is a safe final concentration of GndHCL in the cell cultures?

27 March 2016 4,090 4 View

Could you please suggest a housekeeping gene as a loading control for Western blot from spleen lysates?

Hi Everyone,So I am trying to to do a Western blot from mouse spleen lysates of four different genotypes. With actin I get plenty of background whereas GAPDH works better. The problem is that in...

14 February 2016 3,948 2 View