I am using Betamethasone dipropionate purchased from Sigma. I dissolved them in DMSO instead of Chloroform and they dissolved very well. But when I am adding them as a treatment to the cell suspension (SW 982 and HaCat cell line) its getting milky appearance and crystallizes around the edges of the well. I am also adding DMSO as a negative control but it looks clear. So its might not the DMSO which is making this milky appearance. I am using 5%FBS containing DMEM media for cell harvesting. What should I do now? Should I buy new Betamethasone dipropionate from another company, if so then please suggest me from your practical experience.
According to this reference, the aqueous solubility of betamethasone is 0.05 mg/ml. If you have tried to add it to a final concentration higher than that, it probably crystallized or precipitated.
http://www.drugbank.ca/drugs/DB00443#properties
DMSO is miscible with water, so it will not cause turbidity, unless you added so much that the serum proteins precipitated. I'm not sure how much that would take.
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