Nur Mahammad

6 Questions 5 Answers 0 Followers

Questions related from Nur Mahammad

Can ROS (reactive oxygen species) come out of mitochondria without compromising its membrane? If so how?

I am investigating cellular ROS generation and found an increased amount of ROS while mitochondria was still intact. I am also considering peroxisomes and endoplasmic reticulum as other sources of...

11 October 2022 433 1 View

What does mean by dilution 1:4? Is it 10µl+40µl, or 10µl+30µl?

I am following a potocol from this paper : P8.05 * Selective targeting of CTNBB1-, KRAS- or MYC-driven ... They suggest to mix the stock in 3 different dilutions (1:1, 1:4 and 4:1) but no more...

26 February 2020 9,206 2 View

How stable caspase 3/7 are?

I treated cells with some inhibitors and find them morphologically unhappy after 24 hours. I did Resazurin vaibility assay to measure their viability and find almost 70% reduction. To understand...

03 November 2016 6,155 2 View

I treated HaCat (Keratinocyte cell line) with some inhibitors and they reacted to commit suicide, But I dont understand the death pattern?

The cells are making completely round balloon like structure where the cell remnants accumulate at one corner of the balloon.

09 June 2016 2,555 4 View

Could you please help me sorting out Betamethasone dipropionate use in cell culture system??

I am using Betamethasone dipropionate purchased from Sigma. I dissolved them in DMSO instead of Chloroform and they dissolved very well. But when I am adding them as a treatment to the cell...

02 February 2016 1,615 2 View

What is the standard percentage of viability in the cell subculture?

I was sub culturing one cell line and after 3/4 days. When I count the cell, I find 30% viability. I think something is wrong with my medium or incubation system. Now I wonder what is the standard...

21 November 2014 8,786 10 View