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Questions related from Nur Mahammad
I am investigating cellular ROS generation and found an increased amount of ROS while mitochondria was still intact. I am also considering peroxisomes and endoplasmic reticulum as other sources of...
11 October 2022 467 1 View
I am following a potocol from this paper : P8.05 * Selective targeting of CTNBB1-, KRAS- or MYC-driven ... They suggest to mix the stock in 3 different dilutions (1:1, 1:4 and 4:1) but no more...
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I would really appreciate if someone share his/her experience of inducing TGFb in PBMC and THP1. Please mention inducing agent and condition of media and treatment duration etc. Experience from...
13 August 2018 1,526 2 View
I treated cells with some inhibitors and find them morphologically unhappy after 24 hours. I did Resazurin vaibility assay to measure their viability and find almost 70% reduction. To understand...
03 November 2016 6,180 2 View
The cells are making completely round balloon like structure where the cell remnants accumulate at one corner of the balloon.
09 June 2016 2,582 4 View
I am using Betamethasone dipropionate purchased from Sigma. I dissolved them in DMSO instead of Chloroform and they dissolved very well. But when I am adding them as a treatment to the cell...
02 February 2016 1,640 2 View
I was sub culturing one cell line and after 3/4 days. When I count the cell, I find 30% viability. I think something is wrong with my medium or incubation system. Now I wonder what is the standard...
21 November 2014 8,812 10 View
