Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

Where can I find these two papers?

14 January 2021 1,572 3 View

How should I prepare my tissue sample for biodistribution study?

I want to evaluate the biodistribution of an elastin-based nanoparticle drug in tumor, heart, liver, and kidney for the first time. Can anyone give me any idea about how to prepare my tissue...

04 January 2021 3,661 3 View

How to calculate the normalized frequency V of photonic crystal fiber using COMSOL?In particular, how to calculate NEFF and nFSM using COMSOL?

24 November 2020 967 2 View

Hi every one. Can any body help me in finding PCR based markers for rice fertility restorer genes in R-line?

I need PCR based markers related to fertility restorer genes in R-line of rice hybrid, fertility maintainer genes in B-line of rice and cytoplasmic male sterility genes related markers in -line.

17 November 2020 4,193 1 View

What type of model for Interclass Correlation Coefficient (ICC) analysis should I use to check reliability of test-retest data?

I measured a Psychometric parameter for 60 participants. I collected the data thrice, so I acquired 3 values for that parameter, of which I calculated the mean value. Since the data was relative...

25 October 2020 5,075 3 View

Should you report quality of individual studies in rapid or systematic reviews?

I am sure that I have read guidance that reporting individual quality of studies in reviews is not good practice, but that it is better to report quality of evidence statements (which are based on...

12 October 2020 7,075 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View