I am trying to simulate descriptor (singular) systems with time delays, but I don't know how.Any ideas?

22 November 2016 9,400 1 View

What standard you suggest, to measure the fluorescence quantum yield with excitation wavelength of 400 nm?

I synthesized nanoparticle with fluorescence excitation wavelength of 400 nm and emission wavelength of 450 nm. Therefore, to measure the quantum yield, we should use standards that have an...

12 May 2016 5,809 5 View

Does anyone know how to calculate chemical potential energy of hydrogen or halogens when GNR edges are functionalized with hydrogen or halogen atoms?

14 October 2015 6,859 2 View

I need experimental data for validation of desorption langmuir kinetics model?

29 August 2015 4,068 3 View

Looking for an alternative material that withstand impacts without getting smashed

Hi everyone. We have a musical instrument, that is Constantly being played under sequential impacts in fast pace (similar to dulcimer). To achieve a softer sound, it's common to add a small piece...

01 January 1970 3,235 4 View

Connection between FPGA and Simulink

How can I make a connection between FPGA and Simulink? I want to use FPGA-In-the-Loop (FIL) and my purpose is performing altera board DE1-soc and Simulink. Unfortunately I haven't found a way to...

01 January 1970 9,556 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View