Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

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Melissa Bemrose

You might want to check out these two earlier threads:

David Sherry's answer in this thread is very detailed:

ttps://www.researchgate.net/post/Does_anybody_have_a_standardized_protocol_for_WGAwheat_germ_agglutinin_and_IB4_Isolectin_B4_co-staining_of_mice_whole_heart_sections

Another thread regarding WGA in FFPE tissue sections

https://www.researchgate.net/post/Wheat-Germ-Agglutinin-Staining-WGA-in-FFPE-tissue-sections

I've only used WGA on fixed cells, not sections, so I can't speak from personal experience. Hopefully the 2 threads above will point you in the right direction.

Best of luck!

Melissa

Jinbiao Chen

An interesting question !!

OK, I did a PubMed search for you : )

Here are 2 papers:

1. Brain Struct Funct. 2011 Sep;216(3):159-69. doi: 10.1007/s00429-011-0304-2. Epub 2011 Mar 9. Fluorescence mapping of afferent topography in three dimensions

Stacey L Reeber1, Samrawit A Gebre, Roy V Sillitoe

PMID: 21387082

DOI: 10.1007/s00429-011-0304-2

2. Curr Protoc Neurosci. 2017 Apr 10;79:1.28.1-1.28.24. doi: 10.1002/cpns.28. WGA-Alexa Conjugates for Axonal Tracing

Sabrina L Levy, et al.

PMID: 28398642

PMCID: PMC5830103

DOI: 10.1002/cpns.28

Chapter WGA-Alexa Conjugates for Axonal Tracing: WGA-Alexa Fluoresce...

Best best of Luck