Hi
I am searching for suitable HPLC column for the analysis of beta sitosterol, gamma oryzanol, sesamin, sesamolin, tocopherol and tocotrienols from edible oils such as sesame oil and rice bran oil. I have found articles that used PFP columns and C18 columns. I already got one C18 column (Kinetex 2.6um EVO C18 100A (100 mm x 2.1 mm x 2.6 um) column) but I do not find any publications that used the same column, but there are articles that used C18 columns of other brands.
Few researchers have used Kinetex PFP column (250 mm× 4.6 mm, 5 μm) for the analysis of same compounds from edible oils. So I am wondering whether I will be able to use the C18 column that I already have or not? If not, do I need to use PFP column or can you suggest me any suitable columns for this purpose.
Thanks in advance.
You can use C18 poroshell guard column for phytosterol, tocopherol etc.
We routinely use YMC c30 carotenoids column (https://www.ymc.co.jp/en/columns/ymc_carotenoid/)
for the simultaneous analysis of tocols (tocopherols and tocotrienols), carotenoids, phytosterols, and many other lipophilic compounds.
It can resolve all 4 tocopherols (α-, β-, γ- and δ) and 4 tocotrienols (α-, β-, γ- and δ).
https://doi.org/10.1016/j.indcrop.2020.112441
https://link.springer.com/article/10.1007/s11694-018-9865-6
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