I would like to know for IAV, HIV and HCV the copy number of each of the proteins assembling these mature viruses. Any help/reference answering the question will be greatly appreciated.
HIV roughly 2500 Gag (2000-5000) and for Env 7-20 trimers of Env gp120 non-covalently link to gp41).
For IAV, there are some old estimates in Inglis SC 1976 PMID: 982839 (IIRC) and Ruigrok 1984. Roughly a 1000 or so HAs, rather less NA (couple of hundred) seems to be the consensus, backed up by more modern cryo EM imaging where they just count spikes (Aubrey Harris, Lesley Calder). The uncertainty is over M1, where the various studies disagree, with ranges from 800 or less to 2000 or more. M2 is low, circa 30 or so. The polymerase proteins much less, perhaps as few as 8 copies each. For NP, my notes let me down but ballpark, divide 13000 by 24 for the stoichiometry of genome:site size.
All this is for ~ 100 nm 'spherical' particles of course. The calculations go out of the window for a 10 µm filamentous particle...
Paul
Thanks for the replies. I am interested in all the proteins that compose the mature viruses. I would need a reference for the HCV and HIV estimates. Bye the way, is there any reference about how/what controls protein levels in each of these viruses. Actually, I am going to open posts about this.
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