I am interested in assembling a file with the sequences of the most highly expressed proteins in humans. Are these proteins available somewhere?
Hi Pedro,
Your question is quite broad or I have misinterpreted what you are asking . I would assume you are interested in some tissue specific protein expression levels, not in an entire human as in the entire human body the most highly expressed proteins would be those that are present in a majority of cells such as collagen, actin, tubulin, histones, etc. If you are looking for tissue specific protein expression you can likely find many databases for that including the Human Protein Atlas, see http://www.proteinatlas.org/humanproteome/tissue+specific. Once you finalize the list of the proteins you are interested in, you can find their amino acid sequence by searching a number of databases such as NCBI (https://www.ncbi.nlm.nih.gov/protein).
Good luck!
Some other highly expressed proteins are those that are major components of blood (including albumin and hemoglobin), and muscle (including actin, myosin, and myoglobin). Ribosomal proteins should also be considered.
Thanks for the answers. I understand that some proteins are tissue specific but I am interested in a set of proteins that express at high levels, regardless of tissue and cell but not necessarily on every cell; for example proteins with above 10000 copies per cell. I already considered house keeping genes but they are not necessarily expressed at high levels. In contrast, structural proteins and ribososomal proteins should be included.
@Pedro A Reche
Your question is not so clear. The expression of proteins in any organism differs from region to region. For example, you can observe high expression of alpha-synucleins in substantia nigra and brain regions but you won't be able to see its expression in liver or kidneys.
To have a general idea protein expression, peripheral blood can be sequenced and expression of proteins can be analyzed. But, still, this expression changes from time to time
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