What proteins are most highly expressed in humans?

I am interested in assembling a file with the sequences of the most highly expressed proteins in humans. Are these proteins available somewhere?

06 July 2017 5,761 4 View

Where are impact points?

Impact points have disappeared from Researchgate. Hard to understand this downgrade . Please, get them back. It was a helpful metric that one could not get that easily anywhere else.

03 April 2017 9,969 1 View

Can B-factors be compared between different protein structures?

04 May 2016 7,214 4 View

How to correct negative branches from neighbor-joining method?

01 February 2015 7,760 3 View

Year to report impact factor of an article?

09 October 2014 5,918 4 View

Are there studies on the incidence of autoimmunity in spouses?

Diet and microbiota are two intertwined factors that could act as environmental triggers of systemic autoimmune diseases. Both diet and microbiota are likely to be similar between spouses and I...

03 April 2014 6,641 12 View

Aligning a protein sequence onto a structure-based multiple sequence alignment ?

I am trying to align a sequence to a multiple sequence alignment obtained from an 3D-structural alignment (superimposed 3D structures). The sequence is related to the sequences in the multiple...

04 May 2013 250 16 View

Companies for ordering Custom Synthetic Peptides

We need synthetic peptides for Elispot assays and we wonder from which company to get them. We have found that ChinaPeptides offer peptide synthesis at very competitive prices. However, we have...

03 April 2013 4,177 2 View

Why are clustal and Phylip Bootstrap N-J trees different?

ClustalX has an option to obtain bootstrap neighbor joining trees from an alignment. However, the trees differ considerably from those one can get using bootstrapping with the phylip package...

31 December 2012 2,094 14 View

Isolation of effector T cells from peripheral blood

I am interested in isolating effector T cells from peripheral blood. Does anyone know any recipe/kit or have any recommendation?

09 October 2012 9,301 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View