Conserved miRNA in plants?

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Ujjal Kumar Nath

The attach manuscript might be helpful for you to get your answer:

Magdalena Klimek-Chodacka

Ujjal Kumar Nath Thank you for your response. In the given article was the information that blastn against miRBase was made. Were any additional paramters were set? Can you recommend a specific software?

Ramamoorthy Sankaranarayanan

The following article deals about annotating miRNAs. For detail explanation please ping me.

http://www.plantcell.org/content/20/12/3186.long .

As Ujjal Kumar Nath mentioned, Blast against miRBase repository retrieve the reported miRNAs.

@ Ramamoorthy Sankaranarayanan Thank you for your answer. Should I allow for some mismatch during blastn analysis?

Yes as per Mayer's criteria

Great, thanks. I'm going to read this paper

Cheers

Have you gone through the article? Was that useful to you?

Hi, Thanks for this article. I found that there could be 2 mismatch nucleotide. Do you know a good software for that purpose?

Software for what purpose?

In my PhD, I have predicted and screened plant miRNAs. i am willing to help you. Please be specific in your questions so that I could understand and help you better.