I want to inject a Cre dependent virus to be expressed in pyramidal neurons, and I have Emx1Cre adult mice. I know Emx1 is a developmental gene, but found a paper showing expression of Emx1 also in adults.
I always take the longest time thinking about titles and writing abstracts, so I think it might be a good use. But I wonder if journals should include this in their guidelines, or else, it's not a...
12 February 2024 7,730 1 View
Good evening, Can you please advice on a protocol for labeling of OMVs for cellular uptake assay. OMVs are derived from gram-negative strain. I was thinking of applying R-18 but other dyes are...
03 October 2023 2,929 3 View
Hello, in our lab we use F-AAS for determination of Mg in urine and Li, Zn in serum. Except Zinc QC 2 everything works fine. Is it possible there is problem with zinc controls? I am waiting for...
04 September 2023 4,169 1 View
Hi! Im experiencing problems with CamKII immunostaining for free floating mouse PFC and Hp slices. I ve tried different blocking agents ( NDS, NGS, BSA in different percentages), different mouse...
30 July 2023 7,341 0 View
I performed CRISPR-KO of p53 in fibroblasts. I've got cells that lost the activity of WTp53 (several target genes tested on qPCR) but on western blot, I see still quite a high level of a protein...
15 January 2023 8,080 0 View
How can I quantify DAB using ImageJ or QuPath (staining intensity and number of positive cells)?
03 July 2022 6,002 3 View
I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...
08 June 2022 2,467 0 View
What polymeric material has the most similar properties as nylon 6.6 in terms of the processing and mechanical properties, and it is not from the polyamides family?
05 August 2021 6,193 8 View
I am currently looking for a scale to measure online self-disclosure. The results will help me write my master's thesis.
13 July 2021 9,306 2 View
I'm going to analyze GSK-3 concentration in rat hippocampus homogenate but I don't know how correctly should I calculate this concentration per total protein level examined by Bradford method?
17 May 2021 7,370 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View