How I can add publication with maiden name?

Good morning, can You please tell me how I can add an article that was published under my maiden name? When I try to add an article with this name, it appears information that I should be an...

21 February 2021 6,866 3 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Is it possible for Mesenteric Lymph Nodes (MLNs) murine cells to do Real-time PCR (Q-PCR)?

I need to co-culture MLNs cells from a mouse in 24 well plates with bacteria. I will seed the cells 5x10^5 cells/well with the presence of bacteria 5 to 10 times higher. Is it possible for me to...

15 September 2020 1,163 2 View

Remote Sensing of Seagrass Population?

Hi, I'm trying to find the most effective way with monitoring of seagrass polpulation with using remote sensing. Thank you very much for your help, Magda

15 April 2020 8,796 8 View

KEGG pathway mapping of genes and proteins in prokaryotes?

Hi, I have a list of differentially expressed genes and proteins in mycobacteria and I would like to map them to KEGG pathways - could anyone advise how to go about it?

21 March 2020 1,308 3 View

Can you recommend literature in the impact of rain variability on soil erosion modelling?

In hydrological modelling the impact of spatio-temporal variability on modeled runoff responses is an active research topic (e.g. Sun et al., 2000, Syed et al., 2003, Kim et al., 2008,Vieux et...

21 November 2019 4,650 3 View

Is it possible to change (to extend) the logarithmic scale in x axis in flow cytometry histograms?

I need some information whether it is possible to extend the logarithmic scale (x axis, MFI level) in flow cytometry histograms? In my case the maximal value is 104, which is to low for some...

21 November 2019 3,986 3 View

Is there a device which can dillute a gas-sample before it enters a continious-flow gas-analysator?

I want to measure gaseous NH3 emitted from slurry with the gas-analysator G2508 from Picarro. I expect the concentration to be ten and more times higher than the operating range of the...

02 October 2019 8,276 3 View

Conserved miRNA in plants?

I am asking for help in identifying known miRNAs in plants. After bioinformatic analyzes, I have a list of miRNAs identified and I do not know what kind of criteria adopt in order to find among...

12 June 2019 3,479 10 View

Which statistical tests should I run?

So here's my dilemma. I wanted to run multiple 2 and 3 way anovas on various data sets as well as a multiple regression analysis, but most of them don't meet the assumptions of normality and...

04 June 2019 862 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View