In situ hybridization on cre-recombinased mice protocol?

Has anyone done FISH on cre-recombinased mice? I want to know if anyone has successfully been able to quench the autofluorescence from the those mice to successfully visualize the targeted genes?...

31 December 2019 5,028 3 View

Liquid chromatography mass spectrometry (LC-MS) and condition media analysis?

Has anyone done LCMS on condition media for protein quantification? I have this media (after the exposure of different flow conditions) that i would like to use LCMS for protein quantification...

01 February 2018 1,344 8 View

How can I make potassium free DMEM media?

Any ideas on how to make a potassium free DMEM media or any company that already sells potassium free media without customizing it? I have reached out to few companies and their rates are super...

31 December 2017 1,419 2 View

Porcine vascular endothelial cell isolation - from the aorta?

Hello, Are there any protocols for the isolation and purification of porcine vascular endothelial cells (from the aorta)? I would like to have my study from the primary culture instead of...

09 October 2017 9,578 3 View

RT-qPCR data analysis..(deltadeltaCt method)?

Hello, I have three different groups (Static/A/B) and I want to use delta delta Ct method to show my results in a log form. Previously I always had an untreated control to do the delta delta...

01 February 2017 7,676 3 View

Any good ALPL primer set for porcine?

01 February 2017 386 1 View

How to perform cDNA and primer quality assessments?

Hi, I have recently been trying to run RT-qPCR experiments for interstitial cells. I used Qiagen RNA extraction kit where I got ~500ng/ul of RNA with 260/280~2 and 260/230~1.9, which shows me that...

31 December 2016 7,986 12 View

How to avoid interstitial cell contamination from endothelial cells?

Does anyone know of a method/tip to avoid interstitial cell contamination from endothelial cells? I have been trying to isolate valvular endothelial cells (pig) and keep getting interstitial cell...

11 December 2016 8,311 4 View

Where to start learning molecular biology/signaling pathways?

I want to start learning about different signaling pathways and post transcriptional pathways.. I am not sure where to start. Any help/tip/resources would be greatly appreciated

11 December 2016 4,716 2 View

Any software recommendations for digitizing images?

Does anyone has any software (free) recommendation for digitizing images? I have few echocardiogram images (velocity vs. time) that I would like to digitize. I have been trying to use...

10 November 2016 709 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View