Weber Lin

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Questions related from Weber Lin

Is there any suggestions about MUC5AC protein detection by Western blot?

Hello everyone, I'm trying to detect MUC5AC protein by Western blot. Unfortunately, there was only appears unexpected non-specific bands (picture as attachment). My experimental protocol as...

01 January 2017 1,988 2 View

Can a protein degrade only at the C-terminal?

I want to produce a recombinant protein about 65 kDa by BL21 E.coli and purify by Ni-sepharose beads (GE healthcare). (I fused myc and his tag at C-terminal end of my protein) After elute my...

01 January 2015 2,317 6 View