In situ hybridization on cre-recombinased mice protocol?

Has anyone done FISH on cre-recombinased mice? I want to know if anyone has successfully been able to quench the autofluorescence from the those mice to successfully visualize the targeted genes?...

31 December 2019 5,028 3 View

Liquid chromatography mass spectrometry (LC-MS) and condition media analysis?

Has anyone done LCMS on condition media for protein quantification? I have this media (after the exposure of different flow conditions) that i would like to use LCMS for protein quantification...

01 February 2018 1,344 8 View

How can I make potassium free DMEM media?

Any ideas on how to make a potassium free DMEM media or any company that already sells potassium free media without customizing it? I have reached out to few companies and their rates are super...

31 December 2017 1,419 2 View

Porcine vascular endothelial cell isolation - from the aorta?

Hello, Are there any protocols for the isolation and purification of porcine vascular endothelial cells (from the aorta)? I would like to have my study from the primary culture instead of...

09 October 2017 9,578 3 View

Condition media assays?

Hello, I was wondering if anyone knows different conditioned media assays to quantify protein concentrations besides using ELISA. I have conditioned media from arias treatments and I want to do...

08 September 2017 3,674 2 View

RT-qPCR data analysis..(deltadeltaCt method)?

Hello, I have three different groups (Static/A/B) and I want to use delta delta Ct method to show my results in a log form. Previously I always had an untreated control to do the delta delta...

01 February 2017 7,676 3 View

Any good ALPL primer set for porcine?

01 February 2017 386 1 View

How to perform cDNA and primer quality assessments?

Hi, I have recently been trying to run RT-qPCR experiments for interstitial cells. I used Qiagen RNA extraction kit where I got ~500ng/ul of RNA with 260/280~2 and 260/230~1.9, which shows me that...

31 December 2016 7,986 12 View

How to avoid interstitial cell contamination from endothelial cells?

Does anyone know of a method/tip to avoid interstitial cell contamination from endothelial cells? I have been trying to isolate valvular endothelial cells (pig) and keep getting interstitial cell...

11 December 2016 8,311 4 View

Any software recommendations for digitizing images?

Does anyone has any software (free) recommendation for digitizing images? I have few echocardiogram images (velocity vs. time) that I would like to digitize. I have been trying to use...

10 November 2016 709 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View