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Questions related from Ravi P. Cholia
I rescued my stably Knock-Down cells (passage >15) using its WT and mutant form (flag and myc tagged). Again I selected these cells for stably over expressed cells. However, in this rescue cells...
10 October 2019 800 0 View
I am facing problem in the transfer of high molecular weight proteins to a nitrocellulose membrane from SDS-PAGE gel. Experimental target protein has a molecular mass of ~125kDa, and I tried...
10 October 2015 8,430 7 View
Is there is any possibility that my gel will not suffer any damage/alteration in blotting, if I store the gel before western blotting?
11 November 2014 10,066 9 View
As media contain all the necessary factors for the growth of cells. The excess may result in altered behavior of cells, if I use undetermined cell culture media volume, will it affect the growth...
10 October 2014 8,461 12 View
There is variation in results in every well of culture plate. How can we avoid result variation in cell culture plates? I always seed equal no. of cells, but sometimes they show variation. I...
09 September 2014 1,091 23 View
I am facing a problem during extraction of total proteins from cell lysate. I am using cell lysis (RIPA) buffer with standard composition as described in scientific literature. But every time my...
09 September 2014 1,711 6 View
As for a particular range of treatments we observe a hyperbolic/bell curve. Which region do we select from the graph for experiments? Is there any particular criteria for dose concentration...
08 August 2013 6,506 4 View
