12 Questions 8 Answers 0 Followers
Questions related from Sana Nasim
Has anyone done FISH on cre-recombinased mice? I want to know if anyone has successfully been able to quench the autofluorescence from the those mice to successfully visualize the targeted genes?...
01 January 2020 5,041 3 View
Has anyone done LCMS on condition media for protein quantification? I have this media (after the exposure of different flow conditions) that i would like to use LCMS for protein quantification...
02 February 2018 1,358 8 View
Any ideas on how to make a potassium free DMEM media or any company that already sells potassium free media without customizing it? I have reached out to few companies and their rates are super...
01 January 2018 1,435 2 View
Hello, Are there any protocols for the isolation and purification of porcine vascular endothelial cells (from the aorta)? I would like to have my study from the primary culture instead of...
10 October 2017 9,593 3 View
Hello, I was wondering if anyone knows different conditioned media assays to quantify protein concentrations besides using ELISA. I have conditioned media from arias treatments and I want to do...
09 September 2017 3,687 2 View
Does anyone have a good set of ALPL forward and reverse primer that they have tested out and it works. I have tried out 3 different sets and none of them has properly given me...
02 February 2017 402 1 View
Hello, I have three different groups (Static/A/B) and I want to use delta delta Ct method to show my results in a log form. Previously I always had an untreated control to do the delta delta...
02 February 2017 7,691 3 View
Hi, I have recently been trying to run RT-qPCR experiments for interstitial cells. I used Qiagen RNA extraction kit where I got ~500ng/ul of RNA with 260/280~2 and 260/230~1.9, which shows me that...
01 January 2017 8,002 12 View
I want to start learning about different signaling pathways and post transcriptional pathways.. I am not sure where to start. Any help/tip/resources would be greatly appreciated
12 December 2016 4,730 2 View
Does anyone know of a method/tip to avoid interstitial cell contamination from endothelial cells? I have been trying to isolate valvular endothelial cells (pig) and keep getting interstitial cell...
12 December 2016 8,325 4 View
Does anyone has any software (free) recommendation for digitizing images? I have few echocardiogram images (velocity vs. time) that I would like to digitize. I have been trying to use...
11 November 2016 725 3 View
Hi, Does anyone know of a database for all the genes present at the pig's aortic valve? I am wondering if there is a database for that. I was able to find all the genes expressed in the pig but...
10 October 2016 7,791 0 View
