Hi!
Does anyone have experience with dermal fibroblast culture using collagen-coated flasks? Does the collagen on the flask surface impact the production of collagen by these cells (when compared with standard cell culture flasks)?
Thank you!
I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...
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I need to know the possibilities of equipment for evaluating greenhouse gas emissions in peatlands of different states of conservation. Can anyone help me? Or alternatively, recognized...
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According with the Arrhenius equation the activation energy can be estimated determining the rate constant. Why often we see Arrhenius plots with the ln (reaction rate) as a funtion ln(1/T)?
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Good morning, I am trying to study the antioxidant activity of ZnO NPs. I have already performed some methods, such as DPPH and ABTS assay, but without success. When I search in the literature...
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As a 1st time user of BEAS-2B cells (human bronchial epithelial cells), which I'm starting to culture, I've noticed that they've been growing really slowly .... They were acquired by ATCC no...
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Good morning, I am trying to make the synthesis of ZnO NP with chitosan, but in the end of the process, the sample become like a stone/rock, I cannot destroy to powder, even with a pestle and...
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I did a transformation with a Golden Gate ESP3I reaction in DBH5 e coli. However, I got just one colony and it is very different from what I am used to. Instead of a circled format colony, I get a...
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I have a sample and in my study were going to compare 2 or 3 samples. If I am going to compare 2 groups, I need to know if one of the sample has a normal distribution and another doesn't, can I...
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I have used ethidium bromide solution for genotyping different mouse strains successfully, but the laboratory that I am working on currently does not allow it. Currently, I have been using a...
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I am wondering if these low levels of total RNA the samples are enough for RNA-seq. Does anyone already did it or has any suggestions to get a reliable data for bioinformatic analysis?
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
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Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
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I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
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I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
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