Good morning,
I am trying to study the antioxidant activity of ZnO NPs. I have already performed some methods, such as DPPH and ABTS assay, but without success.
When I search in the literature for methods to measure antioxidant activity of ZnO NPs, the syntheses of these ZnO NPs are biosyntheses (green synthesis), always with extracts of something natural. Which makes me question if it is really the ZnO NPs that have the antioxidant activity or if this activity comes from the extracts used in the syntheses.
Can anyone help me how to measure the antioxidant activity of ZnO NPs that have not been synthesized by natural methods? Does anyone have knowledge on this matter?
Thank you very much.
Ana Rita Mendes
Methods of measuring the antioxidant activity of zinc oxide nanoparticles by chemical and green methods
· 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical inhibition assay
· Measuring the antioxidant activity of zinc oxide nanoparticles by green method with radical absorption method ABTS (2,2-azinobis)3-ethylbenzothiazoline-6-sulfonic acid
· Various chemical methods have been proposed for the synthesis of zinc oxide nanoparticles (ZnO NPs), including the reaction of zinc with alcohol, steam transfer, hydrothermal synthesis, sedimentation method, etc.
· Recently, the use of plants or the green synthesis of nanoparticles has been proposed as a new method that can be an alternative to the synthesis of nanoparticles by chemical and physical methods. Plants and their derivatives, micro-organisms such as bacteria, fungi, algae and yeast are among the proposed biological systems for the green synthesis of nanoparticles. Several fungi such as oxysporum Fusarium Alternaria alternata, Fusarium semi-tectum, Pleurotus, Penicillium brevicompactum, Clostridium, Aspergillus fumigates, sojarcaju versicolor are used for the synthesis of nanoparticles.
Synthesis method of zinc oxide nanoparticles: In order to synthesize zinc oxide nanoparticles, 2 ml of aqueous solution of the extract is added to 50 ml of 0.02 M zinc acetate solution in two aqueous solutions to obtain a uniform solution. Then, the pH of the solution is set to 9 with the help of 0.2 M soda solution and it is strongly stirred on a magnetic stirrer for two hours. The resulting sediment was separated from the solution with the help of filter paper, and after washing 3 times with deionized water and ethanol, it was calcined in a 500-degree oven to obtain zinc oxide nanoparticles.
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