Good morning,

I am trying to study the antioxidant activity of ZnO NPs. I have already performed some methods, such as DPPH and ABTS assay, but without success.

When I search in the literature for methods to measure antioxidant activity of ZnO NPs, the syntheses of these ZnO NPs are biosyntheses (green synthesis), always with extracts of something natural. Which makes me question if it is really the ZnO NPs that have the antioxidant activity or if this activity comes from the extracts used in the syntheses.

Can anyone help me how to measure the antioxidant activity of ZnO NPs that have not been synthesized by natural methods? Does anyone have knowledge on this matter?

Thank you very much.

Ana Rita Mendes

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