Does anyone perform RNA-seq from samples with low levels of RNA (5-10 pg/ul)?

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Abhijeet Singh

You are talking about total RNA and not the depleted RNA that is in itself tells that sample will not be a good choice to go for RNA-Seq

Even with that amount of mRNA, sequencing is always doubtful

Better we not talk about that data analysis, because if rubbish goes in rubbish comes out.

I would not go for RNA-Seq with these samples unless I have a huge amount of funding which I just want to step no matter what...

Natália Ferreira Mendes

Thanks Abhijeet.

Yashika Raheja

Proceeding with such a low amount of sample is not a good choice and specially for bioinformatics you need to be sure about the sequence there should be no error

Thanks Yashika

Xinkun Wang

RNA-seq at this ultra-low level can be done. The SMART-Seq V4 chemistry is designed for this type of samples. Best wishes!

Thanks Dr. Wang. Do you know which company is able to perform RNA-seq with SMART-Seq V4? If you have some indication, please, let me know. My e-mail: [email protected]

Merve Elmastas

Hey did you do smartseq v4 in the end? I have the exact question for my project.