I have used ethidium bromide solution for genotyping different mouse strains successfully, but the laboratory that I am working on currently does not allow it.
Currently, I have been using a GelRed, but my PCR bands are very weak in the gel. I can see (barely) that there is a lot of DNA and I believe that the PCR reaction should be working because of that. So, maybe is something in the gel step.
I'm doing the PCR with GoTaq G2 Green Master Mix, as I used to do before. The primers are the same as the previous. Besides, I can't see the ladder.
Does anybody have any suggestions?
Dear Mendes, you can use non-mutagenic DNA dyes EZ-Vision from Amresco or SafeView from ABM in your gels. Also be careful for DNase contamination in your gels.
Use SYBR Safe DNA Gel stain (Life technologies S33102). This is less hazardous than Ethidium Bromide and can be examined under blue light/UV. We have used it in our laboratory.
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