Hello everyone, I am new to colony formation assay technique. I wanted to know, is there any chemical agent available to induce colony formation in cancer cell lines?
Treat the cells with CHX for 24hrs, collect the floating cells gently and plate for 12 hrs in new medium to see colonies formed by blebbishield emergency program. (Note: VEGFR2 negative cells may not form spheres). No need of soft-agar.